ELISA Mouse Glutamic acid decarboxylase autoanti-IgM (GAD-Ab-IgM)

Reactivity:Mouse (Mus muscµLus) UniProt:N/A Abbreviation:GAD-Ab-IgM Alternative Names:N/A Application:ELISA Range:Detection antibody Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?10.3% Recovery:0.96 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Competitive ELISA Analysis Method??:Qualitative Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAD-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjµgated GAD-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled GAD-Ab-IgM and unlabeled GAD-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GAD-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured. Product Overview:Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD66 wit???? Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).