ELISA Mouse Fibulin-7 (FBLN7)
Reactivity:Mouse (Mus muscµLus)
UniProt:Q501P1
Abbreviation:FBLN7
Alternative Names:DKFZp547D0610; FLJ37440; TM14;
Application:ELISA
Range:0.156-10 ng/mL
Sensitivity:0.053 ng/mL
Intra-AssayCV:?5.6%
Inter-AssayCV:?7.7%
Recovery:0.9
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate FBLN7 in samples. An antibody specific for FBLN7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFBLN7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for FBLN7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBLN7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:FBLN7 encodes a deduced 440-amino acid protein containing a 23-amino acid signal peptide, a Sushi domain, 3 EGF domains with calcium-binding motifs, and a C-terminal fibµLin domain. Fbln7 mRNA was expressed in preodontoblasts and odontoblasts as well as in cartilage, hair follicles, and extraembryonic areas of the placenta.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).