ELISA Mouse Peroxisome Proliferator-activated Receptor beta (PPAR-B)

Reactivity:Mouse (Mus muscµLus) UniProt:P35396 Abbreviation:PPAR-B Alternative Names:FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; PPAR-beta; PPARB; OTTHUMP00000016256|OTTHUMP00000016257|nuclear hormone receptor 1|peroxisome proliferative activated receptor; delta Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.1% Inter-AssayCV:?8.2% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PPAR-B in samples. An antibody specific for PPAR-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAR-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PPAR-B is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAR-B bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Peroxisome Proliferator-Activated Receptor gamma is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPAR-gamma. PPAR-gamma; is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPAR-? plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insµLin resistance, and coronary artery disease. Three known family members are called PPAR alpha, delta, and gamma. Three N-terminal isoforms, called gamma1, gamma 2 and gamma 3, are known to arise by alternative splicing and promoter usage from the PPAR-gamma gene. RXR is an obligate partner for PPAR. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).