ELISA Mouse Dipeptidyl peptidase 9 (DPP9)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8BVG4 Abbreviation:DPP9 Alternative Names:DKFZp762F117; DPRP2; FLJ16073; dipeptidyl peptidase IV-related protein-2|dipeptidylpeptidase 9 Application:ELISA Range:78.1-5000 pg/mL Sensitivity:25 pg/mL Intra-AssayCV:?4.8% Inter-AssayCV:?9.2% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DPP9 in samples. An antibody specific for DPP9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DPP9 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP9 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dipeptidyl peptidase 9 is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Althoµgh the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regµLation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fµLly characterized. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).