ELISA Mouse Insulin-like growth factor-binding protein 7 (IGFBP7)

Reactivity:Mouse (Mus muscµLus) UniProt:Q61581 Abbreviation:IGFBP7 Alternative Names:FSTL2; IGFBP-7; IGFBP-7v; IGFBPRP1; MAC25; PSF; Application:ELISA Range:125.00-8000 pg/mL Sensitivity:47 pg/mL Intra-AssayCV:?4.1% Inter-AssayCV:?6.9% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate IGFBP7 in samples. An antibody specific for IGFBP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIGFBP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for IGFBP7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGFBP7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:By Northern blot analysis, Oh et al. (1996) found that mac25, or IGFBP7, wajs expressed as a 1.1-kb transcript in a broad spectrum of normal tissues. These authors reported that the predicted 277-amino acid protein contains a 26-amino acid signal sequence and the IGFBP motif (GCGCCXXC) at the N terminus. The IGFBP motif is located in a region containing a cluster of 12 conserved cysteines, of which 11 are found in IGFBP7. The amino acid sequences of IGFBP7 and IGFBPs share 20 to 25% identity. Oh et al. (1996) found that recombinant IGFBP7 specifically bound IGFs, althoµgh with relatively low affinity. Kim et al. (1997) proposed that IGFBP7, CTGF , NOV , and IGFBP10 constitute a subfamily of low-affinity IGFBPs. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).