ELISA Mouse Disrupted in schizophrenia 1 protein (DISC1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q811T9 Abbreviation:DISC1 Alternative Names:RP4-730B13.1; C1orf136; FLJ13381; FLJ21640; FLJ25311; FLJ41105; KIAA0457; SCZD9; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.9% Inter-AssayCV:?7.8% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DISC1 in samples. An antibody specific for DISC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DISC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Disrupted-in-Schizophrenia 1 or DISC1 is a protein with a wide array of functions. It participates in cell growth, movement, axonal growth and transport, neural positioning. Disruptions in DISC1 structure may play an important role at least in some cases of schizophrenia, however the evidence from genetic linkage and association studies sµggests that variants of DISC1 do not play a significant role in most cases of schizophrenia, althoµgh DISC1 may be more important in bipolar disorder. In s, this protein is encoded by the DISC1 gene. This gene encodes a protein with mµLtiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development throµgh its interaction with other proteins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).