ELISA Mouse Disks large homolog 4 (DLG4)

Reactivity:Mouse (Mus muscµLus) UniProt:Q62108 Abbreviation:DLG4 Alternative Names:FLJ97752; FLJ98574; PSD95; SAP-90; SAP90; Tax interaction protein 15|discs large homolog 4|post-synaptic density protein 95|synapse-associated protein 90 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?8.1% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLG4 in samples. An antibody specific for DLG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLG4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Disks large homolog 4 is a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromµLtimerizes with DLG2. With DLG2 it is recruited into the same NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a mµLtimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. DLG4 is the best studied member of the MAGUK-family of PDZ domain-containing proteins. Like all MAGUK-family proteins, its basic structure includes three PDZ domains, an SH3 domain, and a guanylate kinase-like domain (GK) connected by disordered linker regions. It is almost exclusively located in the post synaptic density of neurons, and is involved in anchoring synaptic proteins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).