ELISA Mouse Protein delta homolog 1 (DLK1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q09163 Abbreviation:DLK1 Alternative Names:DLK; FA1; PREF1; Pref-1; ZOG; pG2; delta-like 1 homolog|delta-like homolog|fetal antigen 1|preadipocyte factor 1|secredeltin Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.9% Inter-AssayCV:?9.8% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DLK1 in samples. An antibody specific for DLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DLK1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:DLK1 encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thoµght to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).