ELISA Mouse Interleukin-27 subunit beta (EBI3)

Reactivity:Mouse (Mus muscµLus) UniProt:O35228 Abbreviation:EBI3 Alternative Names:IL27B; Epstein-Barr virus induced gene 3|cytokine receptor|interleukin-27 subunit beta Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?8.2% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate EBI3 in samples. An antibody specific for EBI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEBI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for EBI3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EBI3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Epstein-Barr virus induced gene 3was identified by the induction of its expression in B lymphocytes by Epstein-Barr virus infection. The protein encoded by this gene is a secreted glycoprotein, which is a member of the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12). It plays a role in regµLating cell-mediated immune responses.EBI3 is a subunit in 2 distinct heterodimeric cytokines: interleukin-27 (IL27) and IL35. IL27 is composed of p28 (IL27) and EBI3. IL27 can trigger signaling in T cells, B cells, and myeloid cells. IL35, an inhibitory cytokine involved in regµLatory T-cell function, is composed of EBI3 and the p35 subunit of IL12.Interleukin-27 (IL-27)is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (IL-27B) and IL27-p28 (known as IL-30). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).