ELISA Mouse Follicle-stimulating hormone (FSH)

Reactivity:Mouse (Mus muscµLus) UniProt:N/A Abbreviation:FSH Alternative Names:N/A Application:ELISA Range:3.125-200 ng/mL Sensitivity:1.25 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?6.6% Recovery:0.85 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for FSH is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability: