ELISA Mouse Kallikrein-7 (KLK7)

Reactivity:Mouse (Mus muscµLus) UniProt:Q91VE3 Abbreviation:KLK7 Alternative Names:PRSS6; SCCE; kallikrein 7 (chymotryptic; stratum corneum)|protease; serine; 6|signal protein|stratum corneum chymotryptic enzyme Application:ELISA Range:78.1-5000 pg/mL Sensitivity:32 pg/mL Intra-AssayCV:?3.6% Inter-AssayCV:?7.9% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK7 in samples. An antibody specific for KLK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KLK7 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK7 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Kallikrein-related peptidase 7, also known as KLK7, Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence sµggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thoµght to be involved in the proteolysis of intercellµLar cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene resµLts in two transcript variants encoding the same protein. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).