ELISA Mouse Keratin, type I cytoskeletal 18 (KRT18)

Reactivity:Mouse (Mus muscµLus) UniProt:P05784 Abbreviation:KRT18 Alternative Names:CYK18; K18; cell proliferation-inducing protein 46|cytokeratin 18 Application:ELISA Range:78.1-5000 pg/mL Sensitivity:32 pg/mL Intra-AssayCV:?4.2% Inter-AssayCV:?7.1% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KRT18 in samples. An antibody specific for KRT18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KRT18 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT18 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. keratin 18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adµLts to a variety of simple epithelial tissues. Endo B and its coexpressed type II keratin, Endo A (the mouse equivalent of keratin 8), appear to be the first IF proteins expressed during murine development. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).