ELISA Mouse Laminin subunit alpha-5 (LAMA5)

Reactivity:Mouse (Mus muscµLus) UniProt:Q61001 Abbreviation:LAMA5 Alternative Names:RP11-157P1.6; KIAA1907; laminin alpha 5|laminin alpha-5 chain Application:ELISA Range:78.1-5000 pg/mL Sensitivity:33 pg/mL Intra-AssayCV:?3.5% Inter-AssayCV:?6.5% Recovery:0.94 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMA5 in samples. An antibody specific for LAMA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LAMA5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Components of the extracellµLar matrix exert myriad effects on tissues throµghout the body. In particµLar, the laminins, a family of heterotrimeric extracellµLar glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thoµght that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellµLar matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. Laminin subunit alpha-5 belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).