ELISA Mouse Low-density lipoprotein receptor (LDLR)

Reactivity:Mouse (Mus muscµLus) UniProt:P35951 Abbreviation:LDLR Alternative Names:FH; FHC; LDLCQ2; LDL receptor|low-density lipoprotein receptor class A domain-containing protein 3 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.058 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.1% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LDLR in samples. An antibody specific for LDLR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDLR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LDLR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDLR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). Brown and Goldstein won a Nobel Prize for their identification of the Low Density Lipoprotein (LDL) receptor in 1985 whilst they were studying familial hypercholesterolemia. The LDL receptor can be described as a chimeric protein. It is made up of a number of functionally distinct domains that can function independently of each other. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).