ELISA Mouse Left-right determination factor 2 (LEFTY2)

Reactivity:Mouse (Mus muscµLus) UniProt:P57785 Abbreviation:LEFTY2 Alternative Names:EBAF; LEFTA; LEFTYA; MGC46222; TGFB4; endometrial bleeding associated factor|endometrial bleeding associated factor (left-right determination; factor A; transforming growth factor beta superfamily)| Application:ELISA Range:15.6-1000 pg/mL Sensitivity:5.5 pg/mL Intra-AssayCV:?5.4% Inter-AssayCV:?8.6% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LEFTY2 in samples. An antibody specific for LEFTY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEFTY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LEFTY2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEFTY2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Lefty proteins are signaling ligands that act in opposition to the NODAL protein. During vertebrate development they regµLate the degree of left-right asymmetry by controlling the spatiotemporal influence of the NODAL protein.It is involved in embryogenesis and left-right patterning. There are many differences between the left and right sides, including heart and lung positioning. Mutations in these genes cause incorrect positioning of these organs (e.g., situs inversus). Lefty1 in the ventral midline prevents the Cerebrus (paracrine factor or "Caronte") signal from passing to the right side of the embryo.The role of Lefty1 is to restrict the expression of Lefty2 and Nodal to the left side, and that Lefty2 or Nodal encode a signal for 'leftness.' Lefty2 serves as a feedback inhibitor to restrict the range of nodal signaling during establishment of the left-right axis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).