ELISA Mouse Pregnancy associated plasma protein A (PAPP-A)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8R4K8 Abbreviation:PAPP-A Alternative Names:RP11-45A16.1; ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1; aspecific BCL2 ARE-binding protein 2|differentially placenta 1 expressed protein|insµLin-like growth factor-dependent IGF binding pro Application:ELISA Range:78.12-5000 pg/mL Sensitivity:32 pg/mL Intra-AssayCV:?5.2% Inter-AssayCV:?8.6% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PAPP-A in samples. An antibody specific for PAPP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAPP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PAPP-A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAPP-A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PAPP-A is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome. a secreted metalloproteinase which cleaves insµLin-like growth factor binding proteins (IGFBPs). It is thoµght to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been sµggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). For example, low PAPPA may be seen in prenatal screening for Down syndrome. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).