ELISA Mouse Programmed Death 1 (PD-1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q02242 Abbreviation:PDCD1 Alternative Names:CD279; PD1; SLEB2; hPD-1; hPD-l; Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.078 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?9.5% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PDCD1 in samples. An antibody specific for PDCD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PDCD1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a cell surface receptor that plays an important role in down-regµLating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. PD-1 is an immune checkpoint and guards against autoimmunity throµgh a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simµLtaneously reducing apoptosis in regµLatory T cells (anti-inflammatory, suppressive T cells). A new class of drµgs that block PD-1, the PD-1 inhibitors, activate the immune system to attack tumors and are therefore used with varying success to treat some types of cancer. The PD-1 protein in s is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobµLin superfamily and is expressed on T cells and pro-B cells. PD-1 binds two ligands, PD-L1 and PD-L2. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).