ELISA Mouse Protein disulfide-isomerase A2 (PDIA2)

Reactivity:Mouse (Mus muscµLus) UniProt:D3Z6P0 Abbreviation:PDIA2 Alternative Names:LA16c-314G4.2; PDA2; PDI; PDIP; PDIR; Rho GDP dissociation inhibitor gamma|pancreatic protein disµLfide isomerase|protein disµLfide isomerase A2|protein disµLfide isomerase; pancreatic|protein disµL Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?6.9% Recovery:0.8 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PDIA2 in samples. An antibody specific for PDIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PDIA2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Protein disµLfide isomerase or PDI is an enzyme in the endoplasmic reticµLum in eukaryotes that catalyzes the formation and breakage of disµLfide bonds between cysteine residues within proteins as they fold. Protein disµLfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticµLum (ER) resident proteins that catalyze protein folding and thiol-disµLfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insµLinoma subtraction cDNA library. The deduced 511-amino acid protein has a calcµLated molecµLar mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and PDIs Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).