ELISA Mouse Perforin-Pore-forming protein (PF-PFP)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity:Mouse (Mus muscµLus) UniProt:P10820 Abbreviation:PRF1 Alternative Names:RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759|cytolysin|lymphocyte pore forming protein|perforin 1 Application:ELISA Range:7.8-500 pg/mL Sensitivity:1.95 pg/mL Intra-AssayCV:≤5.3% Inter-AssayCV:≤9.8% Recovery:0,99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Pore-forming proteins (Also known as Pore-forming toxins) are protein exotoxins, usually produced by bacteria, such as C.septicum and S.aureus. They are frequently cytotoxic (i.e., they kill cells), as they create unregµLated pores in the membrane of targeted cells Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).