ELISA Mouse Proline-rich membrane anchor 1 (PRIMA1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q810F0 Abbreviation:PRIMA1 Alternative Names:PRIMA; OTTHUMP00000180666|acetylcholinesterase membrane anchor precursor PRiMA Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?6.7% Inter-AssayCV:?8.3% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRIMA1 in samples. An antibody specific for PRIMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRIMA1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscµLar junction and to the membrane of neuronal synapses. collagen Q (ColQ) anchors AChE at the neuromuscµLar junction.PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain. PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. AChE is actually anchored in neural cell membranes throµgh its interaction with PRiMA. PRiMA anchors AChE in mammalian brain and muscle cell membranes. Required to anchor acetylcholinesteras Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).