ELISA Mouse PX domain-containing protein kinase-like protein (PXK)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8BX57 Abbreviation:PXK Alternative Names:FLJ20335; MONaKA; PX ser/thr kinase v2|PX serine/threonine kinase|modµLator of Na;K-ATPase long form Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.8% Inter-AssayCV:?9.3% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PXK in samples. An antibody specific for PXK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PXK is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXK bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The deduced 578-amino acid PXK and 581-amino acid mouse Pxk share over 90% amino acid identity. PXK contains an N-terminal PX-like domain predicted to bind phosphoinositides, a putative protein kinase domain in the central region, which has greatest similarity to Drosophila slob, and a number of polyproline motifs. The authors identified a truncated PXK isoform in both and mouse. RT-PCR analysis detected expression of the fµLl-length Pxk in all mouse tissues examined and expression of the Pxk short isoform in all mouse tissues except skeletal muscle. Immunohistochemical studies detected Pxk expression throµghout the nervous system in both mouse neurons and glia, including oligodendrocytes, astrocytes, microglia, and Schwann cells. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).