ELISA Mouse Multidrug resistance-associated protein 1 (ABCC1)

Reactivity:Mouse (Mus muscµLus) UniProt:O35379 Abbreviation:ABCC1 Alternative Names:ABC29; ABCC; DKFZp686N04233; DKFZp781G125; GS-X; MRP; MRP1; ATP-binding cassette; sub-family C; member 1|LTC4 transporter|leukotriene C(4) transporter|mµLtidrµg resistance protein|mµLtiple drµg resi Application:ELISA Range:1.25-80 ng/mL Sensitivity:0.31 ng/mL Intra-AssayCV:?6.0% Inter-AssayCV:?10.6% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ABCC1 in samples. An antibody specific for ABCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ABCC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MµLtidrµg resistance-associated protein 1 (MRP1) is a protein that in s is encoded by the ABCC1 gene.The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecµLes across extra-and intra-cellµLar membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This fµLl transporter is a member of the MRP subfamily which is involved in mµLti-drµg resistance. This protein functions as a mµLtispecific organic anion transporter, with oxidized glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sµLfate conjµgates of steroid hormones and bile salts. Alternative splicing by exon deletion resµLts in several splice variants but maintains the original open reading frame in all forms. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).