ELISA Mouse Protein Z-dependent protease inhibitor (SERPINA10)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8R121 Abbreviation:SERPINA10 Alternative Names:PZI; ZPI; protein Z-dependent protease inhibitor|protein Z-dependent protease inhibitorprecursor|serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 10 Application:ELISA Range:0.94-60 ng/mL Sensitivity:0.38 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?9.5% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINA10 in samples. An antibody specific for SERPINA10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPINA10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Protein Z-dependent protease inhibitor is a protein circµLating in the blood which inhibits factors Xa and XIa of the coagµLation cascade. It is a member of the class of the serine protease inhibitors (serpins). Its name implies that it requires protein Z, another circµLating protein, to function properly, but this only applies to its inhibition of factor X. It is about 72 kDa heavy and 444 amino acids large. It is produced by the liver.Water et al. found deficiency of ZPI in 4.4% of a cohort of patients with thrombophilia (a tendency to thrombosis).Han et al. first described ZPI in 1998. The same group further characterised it in 2000. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).