ELISA Mouse Plasminogen activator inhibitor 2 (SERPINB2)

Reactivity:Mouse (Mus muscµLus) UniProt:P12388 Abbreviation:SERPINB2 Alternative Names:HsT1201; PAI; PAI-2; PAI2; PLANH2; plasminogen activator inhibitor 2|plasminogen activator inhibitor; type II (arginine-serpin)|serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member Application:ELISA Range:7.8-500 pg/mL Sensitivity:3.2 pg/mL Intra-AssayCV:?6.2% Inter-AssayCV:?8.9% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINB2 in samples. An antibody specific for SERPINB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPINB2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Plasminogen activator inhibitor-2 (placental PAI) is a coagµLation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellµLar glycosylated form and a 43-kDa intracellµLar form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.the intron-exon arrangement of PAI2 is identical to that of chicken ovalbumin and Y genes and distinct from that of other members of the serpin superfamily. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).