ELISA Mouse Heparin cofactor 2 (SERPIND1)

Reactivity:Mouse (Mus muscµLus) UniProt:P49182 Abbreviation:SERPIND1 Alternative Names:D22S673; HC2; HCF2; HCII; HLS2; LS2; OTTHUMP00000198451|heparin cofactor II|leuserpin 2|serine (or cysteine) proteinase inhibitor; clade D (heparin cofactor); member 1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.069 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?7.9% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPIND1 in samples. An antibody specific for SERPIND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPIND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SERPIND1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPIND1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Heparin cofactor II, a protein encoded by the SERPIND1 gene, is a coagµLation factor that inhibits IIa, and is a cofactor for heparin and dermatan sµLfate ("minor antithrombin"). The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sµLfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. Heparin Cofactor II deficiency can lead to increased thrombin generation and a hypercoagµLable state. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).